Ersity of Chinese Medicine, Department of Nephrology, Beijing, China; 4 Xiluoyuan community health service center of Fengtai distict, Department of Traditional Chinese Medicine, Beijing, China Correspondence: Fengxian Meng BMC Complementary and Alternative Medicine 2017, 17(Suppl 1):P120 Objective The objective of this study was to investigate the effect of Qinlingye extract (QLYE) on the inflammatory signal pathway of NLRP3/ TLR4/ NF-B in HKC cells. Methods HKC cells were cultured and induced by uric acid (UA) in model group. While stimulated by UA, the administered groups were intervened by high-, middle-, low-dose of QLYE (1000, 500, 250 umol/L). After 24, 36, 48 h of intervention, the total RNA and protein were extracted. PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/19118039 RT-PCR was used to detect the mRNA transcription of NLRP3, TLR4 and NF-B, Western blot was used to detect the protein expression of NLRP3, TLR4 PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/1669444 and IB. Results 1. Compared with the control group, the mRNA transcription of NLRP3 at 24 h, TLR4 at 36 h and NF-B at 36, 48 h were higher in model group (P < 0.05); the protein expression of NLRP3 and TLR4 were higher, IB was lower at 24, 36, 48 h in model group (P < 0.05, P < 0.01).2. Compared with the model group, the mRNA transcription of NLRP3 in three administered groups at 24 h, TLR4 in high-, middle-dose group at 36 h, and NF-B in high-dose group at 36, 48 h were lower (P < 0.05, P < 0.01); the protein expression of NLRP3 in high-, middle-dose group and TLR4 in high-, low-dose group were lower, IB in high-, middle-dose group were higher at 24 h, the protein expression of NLRP3 in three administered groups and TLR4 in high-, middle-dose group were lower, IB in Sp-cAMPS high-, middle-dose group were higher at 36 h, the protein expression of NLRP3 in middle-dose group was lower, IB in middle-dose group was higher at 48 h (P < 0.05, P < 0.01). Conclusion QLYE may inhibit the NLRP3/ TLR4/ NF-B signal pathway to ameliorate the renal immune inflammatory injuries induced by UA. P121 Effectiveness of mindfulness- and relaxation-based eHealth interventions for patients with medical conditions: a systematic review Michael Mikolasek, Jonas Berg, Claudia Witt, J gen Barth Institute for Complementary and Integrative Medicine, UniversityHospital Zurich, Zurich, 8091, Switzerland Correspondence: Michael Mikolasek BMC Complementary and Alternative Medicine 2017, 17(Suppl 1):P121 Purpose This systematic review aims to summarize eHealth studies with mindfulness- and relaxation-based interventions for medical conditions and to determine if eHealth interventions have positive effects on health. Methods A comprehensive search of five databases was conducted for all available studies from 1990 to 2015. Studies were included if the intervention was mainly technology delivered, included a mindfulness- or relaxation-based intervention strategy and if patients with a medical condition were treated. Treatment effects were summarized by vote counting for different outcomes. Results A total of 2383 records were identified whereof 17 studies with 1855 patients were included in this systematic review. These studies were conducted in patients with irritable bowel syndrome, chronic fatigue syndrome, cancer, chronic pain, surgery, and hypertension. All but one study were delivered online through a web-platform. One study delivered the intervention with iPods. The studies indicate that mindfulnessand relaxation-based eHealth interventions can have positive effects on patients' general health and psychological.